SDRF template for human gut metaproteomics. Extends metaproteomics with host-associated columns aligned with the GSC MIxS human-gut extension (0016004). Combine with ms-proteomics for MS acquisition columns.
The human-gut template adds host-associated metadata fields from the MIxS
human-gut extension. These columns describe the host organism, its health
status, diet, and other clinical context relevant to gut microbiome studies.
PRIDE ontology terms (PRIDE:0000674 children) include MIxS cross-references.
Usage: human-gut + ms-proteomics
| Column | Requirement | Source | Description | |
|---|---|---|---|---|
| source name[sample name] | required | human-gut | Reference to the Sample. This term is used for cross-reference samples in SDRF for proteomics (MI... | |
|
Reference to the Sample. This term is used for cross-reference samples in SDRF for proteomics (MIXS:0001107).
identifier
sample name identifier
Sample_1, Sample_2
|
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| characteristics[host organism] | required | human-gut | Host organism for host-associated microbiome samples | |
|
Host organism for host-associated microbiome samples
ontology
— ontologies: ncbitaxon
Should be a valid NCBI Taxonomy term
Homo sapiens
|
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| characteristics[host subject id] | recommended | human-gut | De-identified unique identifier for the host subject. Corresponds to MIxS host_subject_id (MIXS:0000861). | |
|
De-identified unique identifier for the host subject. Corresponds to MIxS host_subject_id (MIXS:0000861).
identifier
Host subject identifier
subject_001, patient_A, anonymized
|
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| characteristics[host disease status] | recommended | human-gut | Host disease diagnoses. Corresponds to MIxS host_disease_stat (MIXS:0000031). | |
|
Host disease diagnoses. Corresponds to MIxS host_disease_stat (MIXS:0000031).
ontology
— ontologies: mondo, doid
Should be a valid disease ontology term
inflammatory bowel disease, colorectal cancer, healthy
|
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| characteristics[host body site] | recommended | human-gut | Body site where sample was obtained. Corresponds to MIxS host_body_site (MIXS:0000867). | |
|
Body site where sample was obtained. Corresponds to MIxS host_body_site (MIXS:0000867).
ontology
— ontologies: uberon, bto
Body site should be a valid UBERON or BTO term
stool, oral cavity, colon
|
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| characteristics[host genotype] | recommended | human-gut | Observed genotype MIXS:0000365. | |
|
Observed genotype MIXS:0000365.
ontology
— ontologies: efo, gene
should be a valid genotype ontology term
homozygous, heterozygous
|
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| characteristics[host phenotype] | recommended | human-gut | Phenotype of human or other host MIXS:0000274. | |
|
Phenotype of human or other host MIXS:0000274.
ontology
— ontologies: pato, hp
Phenotype should be a valid PATO or HP term
polydactyly, arachnodactyly
|
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| characteristics[host age] | optional | human-gut | Age of host at the time of sampling. Corresponds to MIxS host_age (MIXS:0000255). | |
|
Age of host at the time of sampling. Corresponds to MIxS host_age (MIXS:0000255).
pattern
— pattern:
^\d+[YMWDH]$
Age in standard format (Y=year, M=month, W=week, D=day, H=hour)
45Y, 8W, 3M
|
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| characteristics[host sex] | optional | human-gut | Sex of the host organism. Corresponds to MIxS host_sex (MIXS:0000811). | |
|
Sex of the host organism. Corresponds to MIxS host_sex (MIXS:0000811).
values
— values:
male, female, intersex
Host sex
|
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| characteristics[host body-mass index] | optional | human-gut | Body mass index (weight/height^2). Corresponds to MIxS host_body_mass_index (MIXS:0000317). | |
|
Body mass index (weight/height^2). Corresponds to MIxS host_body_mass_index (MIXS:0000317).
pattern
— pattern:
^[\d.]+$
BMI numeric value
22.5, 30.1, 18.5
|
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| characteristics[host height] | optional | human-gut | Height of the host. Corresponds to MIxS host_height (MIXS:0000264). | |
|
Height of the host. Corresponds to MIxS host_height (MIXS:0000264).
number_with_unit
Host height with unit
175 cm, 1.75 m
|
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| characteristics[host total mass] | optional | human-gut | Total mass of the host. Corresponds to MIxS host_tot_mass (MIXS:0000263). | |
|
Total mass of the host. Corresponds to MIxS host_tot_mass (MIXS:0000263).
number_with_unit
Host mass with unit
70 kg, 85 kg
|
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| characteristics[ethnicity] | optional | human-gut | Ethnicity of the host. Corresponds to MIxS ethnicity (MIXS:0000895). | |
|
Ethnicity of the host. Corresponds to MIxS ethnicity (MIXS:0000895).
pattern
— pattern:
^.+$
Ethnicity description
European, East Asian, African
|
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| characteristics[host diet] | optional | human-gut | Diet type of the host. Corresponds to MIxS host_diet (MIXS:0000869). | |
|
Diet type of the host. Corresponds to MIxS host_diet (MIXS:0000869).
pattern
— pattern:
^.+$
Diet description
omnivore, vegan, western diet, high-fiber
|
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| characteristics[special diet] | optional | human-gut | Special dietary restrictions. Corresponds to MIxS special_diet (MIXS:0000905). | |
|
Special dietary restrictions. Corresponds to MIxS special_diet (MIXS:0000905).
pattern
— pattern:
^.+$
Special diet description
gluten-free, low FODMAP, ketogenic
|
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| characteristics[host last meal] | optional | human-gut | Content of last meal and time since feeding. Corresponds to MIxS host_last_meal (MIXS:0000870). | |
|
Content of last meal and time since feeding. Corresponds to MIxS host_last_meal (MIXS:0000870).
pattern
— pattern:
^.+$
Last meal description
breakfast 4 hours prior, fasting 12 hours
|
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| characteristics[host family relationship] | optional | human-gut | Relationships to other hosts in the same study; can include multiple relationships MIXS:0000872. | |
|
Relationships to other hosts in the same study; can include multiple relationships MIXS:0000872.
pattern
— pattern:
^.+$
family relationship description
father, daughter
|
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| characteristics[host occupation] | optional | human-gut | Most frequent job performed by subject MIXS:0000896. | |
|
Most frequent job performed by subject MIXS:0000896.
pattern
— pattern:
^.+$
occupation description
teacher, engineer
|
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| characteristics[gastrointestinal tract disorder] | optional | human-gut | History of GI tract disorders. Corresponds to MIxS gastroint_disord (MIXS:0000280). | |
|
History of GI tract disorders. Corresponds to MIxS gastroint_disord (MIXS:0000280).
pattern
— pattern:
^.+$
GI disorder description
Crohn's disease, ulcerative colitis, irritable bowel syndrome, none
|
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| characteristics[liver disorder] | optional | human-gut | History of liver disorders. Corresponds to MIxS liver_disord (MIXS:0000282). | |
|
History of liver disorders. Corresponds to MIxS liver_disord (MIXS:0000282).
pattern
— pattern:
^.+$
Liver disorder description
none, fatty liver disease, hepatitis
|
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| characteristics[antibiotic treatment] | optional | human-gut | Recent antibiotic exposure of the host | |
|
Recent antibiotic exposure of the host
pattern
— pattern:
^.+$
Antibiotic treatment description
none, amoxicillin 7 days prior, broad-spectrum
|
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| characteristics[ihmc medication code] | optional | human-gut | Medication codes (IHMC). Corresponds to MIxS ihmc_medication_code (MIXS:0000884). | |
|
Medication codes (IHMC). Corresponds to MIxS ihmc_medication_code (MIXS:0000884).
pattern
— pattern:
^.+$
Medication code(s)
none, A02BC01, N02BE01
|
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| characteristics[host body product] | optional | human-gut | Substance produced by the body where sample was obtained. Corresponds to MIxS host_body_product (... | |
|
Substance produced by the body where sample was obtained. Corresponds to MIxS host_body_product (MIXS:0000888).
pattern
— pattern:
^.+$
Body product description
stool, mucus, saliva
|
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| characteristics[host body temperature] | optional | human-gut | Core body temperature at sample collection. Corresponds to MIxS host_body_temp (MIXS:0000874). | |
|
Core body temperature at sample collection. Corresponds to MIxS host_body_temp (MIXS:0000874).
number_with_unit
Body temperature in Celsius
36.6 °C, 37.2 °C
|
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| characteristics[perturbation] | optional | human-gut | Type of perturbation applied. Corresponds to MIxS perturbation (MIXS:0000754). | |
|
Type of perturbation applied. Corresponds to MIxS perturbation (MIXS:0000754).
pattern
— pattern:
^.+$
Perturbation description
antibiotic administration, dietary intervention, none
|
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| characteristics[chemical administration] | optional | human-gut | Chemical compounds administered to the host. Corresponds to MIxS chem_administration (MIXS:0000751). | |
|
Chemical compounds administered to the host. Corresponds to MIxS chem_administration (MIXS:0000751).
pattern
— pattern:
^.+$
Chemical administration description
metformin 500mg daily, probiotics
|
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| characteristics[host pulse] | optional | human-gut | Resting pulse, measured as beats per minutes (MIXS:0000333). | |
|
Resting pulse, measured as beats per minutes (MIXS:0000333).
number_with_unit
Body pulse in bpm
65 bpm, 72 bpm, 85 bpm
|
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| characteristics[observed host symbionts] | optional | human-gut | The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic s... | |
|
The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host (MIXS:0001298).
ontology
|
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| characteristics[medical history performed] | optional | human-gut | Whether full medical history was collected (MIXS:0000897). | |
|
Whether full medical history was collected (MIXS:0000897).
pattern
— pattern:
^.+$
Medical history performed description
45 year old male with a 2 week history of stomach pain.
|
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| characteristics[project name] | optional | human-gut | Name of the project within which the sequencing was organized (MIXS:0000092). | |
|
Name of the project within which the sequencing was organized (MIXS:0000092).
identifier
project name identifier
Human_microbiome_project
|
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| comment[sample storage temperature] | optional | human-gut | Temperature at which sample was stored (MIXS:0000110). | |
|
Temperature at which sample was stored (MIXS:0000110).
number_with_unit
temperature in Celsius
-80 °C, -20 °C
|
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| comment[sample storage location] | optional | human-gut | Location at which sample was stored (MIXS:0000755). | |
|
Location at which sample was stored (MIXS:0000755).
pattern
— pattern:
^.+$
storage location description
freezer, room
|
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| comment[sample storage duration] | optional | human-gut | Duration for which the sample was stored (MIXS:0000116). | |
|
Duration for which the sample was stored (MIXS:0000116).
pattern
— pattern:
^.+$
storage duration description
40 days, 6 months
|
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| comment[sample volume or weight for DNA extraction] | optional | human-gut | Volume (ml) or mass (g) of total collected sample processed for DNA extraction (MIXS:0000111). | |
|
Volume (ml) or mass (g) of total collected sample processed for DNA extraction (MIXS:0000111).
number_with_unit
volume or weight of DNA extraction
1 ml, 2 g
|
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| comment[organism count] | optional | human-gut | Total cell count of any organism per gram, volume, or area of sample, including count (MIXS:0000103). | |
|
Total cell count of any organism per gram, volume, or area of sample, including count (MIXS:0000103).
number_with_unit
organism count in CFU/ml
500 CFU/ml, 2000 CFU/ml
|
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| comment[oxygenation status of sample] | optional | human-gut | Oxygenation status of sample (MIXS:0000753). | |
|
Oxygenation status of sample (MIXS:0000753).
number_with_unit
Oxygenation status of sample in mmHg
80 mmHg, 100 mmHg
|
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| comment[miscellaneous parameter] | optional | human-gut | Any other measurement performed or parameter collected, that is not listed here (MIXS:0000752). | |
|
Any other measurement performed or parameter collected, that is not listed here (MIXS:0000752).
pattern
— pattern:
^.+$
miscellaneous parameter description
none
|
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